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Cell Signaling Technology Inc cell signaling technology 4060s
Cell Signaling Technology 4060s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 17147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/xp+rabbit+mab+cell+signaling+technology/Phospho-Akt+(Ser473)+XP+Rabbit+mAb/pm41933297-51-36-36
Average 99 stars, based on 17147 article reviews

cell signaling technology 4060s - by Bioz Stars, 2026-09

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Article Title: TM4SF5-mediated abnormal food-intake behavior and apelin expression facilitate non-alcoholic fatty liver disease features.
Article Snippet: Anti-a-tubulin (TU-02) Santa Cruz Biotechnology Cat# sc-8035, RRID:AB_628408 Anti-b-actin (C4) Santa Cruz Biotechnology Cat# sc-47778, RRID:AB_626632 Mouse anti-Osteopontin/SPP-1 R and D Systems Cat# AF808, RRID:AB_2194992 Anti-apln/Apelin rabbit pAb Abcam Cat# ab125213, RRID:AB_10999708 Monoclonal anti-Flag M2 antibody Sigma-Aldrich Cat# F1804, RRID:AB_262044 Mouse anti-Akt1 mAb Santa Cruz Biotechnology Cat# sc-81434, RRID:AB_1118808 Anti-phospho-Akt1 (Ser473) rabbit pAb Santa Cruz Biotechnology Cat# sc-33437, RRID:AB_2225021 Anti-Elk1 rabbit pAb Cell Signaling Technology Cat# 9182, RRID:AB_2277936 Anti-phospho-Elk1 (Ser383) rabbit pAb Thermo Fisher Scientific Cat# PA5-17881, RRID:AB_10980224 Anti-Tm4sf5 (C-terminus) rabbit pAb Homemade: Jung et al.19 https://pubmed.ncbi.nlm.nih.gov/36063136/ Anti-Srebp1 mouse mAb Santa Cruz Biotechnology Cat# sc-365513, RRID:AB_10843812 Anti-Srebp2 mouse mAb Santa Cruz Biotechnology Cat# sc-271616, RRID:AB_10708843 Anti-Fasn (C20G5) rabbit mAb Cell Signaling Technology Cat# 3180, RRID:AB_2100796 Anti-ACC rabbit mAb Cell Signaling Technology Cat# 33676, RRID:AB_2616592 Anti-phospho-ACC (Ser79) rabbit mAb Cell Signaling Technology Cat# 11818, RRID:AB_2687505 Anti-SCD1 (S-15) goat pAb Santa Cruz Biotechnology Cat# sc-14719, RRID:AB_656063 Anti-Dgat2 (C-15) goat pAb Santa Cruz Biotechnology Cat# sc-32400, RRID:AB_2090818 Anti-Acox1 rabbit pAb Proteintech Cat# 10957-1-AP, RRID:AB_2221670 Anti-LC3B rabbit pAb Cell Signaling Technology Cat# 2775, RRID:AB_915950 Anti-p62/SQSTM1 rabbit pAb Cell Signaling Technology Cat# 5114, RRID:AB_10624872 Anti-mTOR Cell Signaling Technology Cat# 2972, RRID:AB_330978 Anti-phospho-mTOR (Ser2448) (D9C2) rabbit mAb Cell Signaling Technology Cat# 5536, RRID:AB_10691552 Anti-phospho-mTOR (Ser2481) rabbit pAb Cell Signaling Technology Cat# 2974, RRID:AB_2262884 Anti-AMPK (D63G4) rabbit mAb Cell Signaling Technology Cat# 5832, RRID:AB_10624867 Anti-phospho-AMPKa (Thr172) (40H9) rabbit mAb Cell Signaling Technology Cat# 2535, RRID:AB_331250 Anti-Cpt1a (D3B3) rabbit mAb Cell Signaling Technology Cat# 12252, RRID:AB_2797857 Anti-Ulk1 (D8H5) rabbit mAb Cell Signaling Technology Cat# 8054, RRID:AB_11178668 Anti-phospho-Ulk1 (Ser555) rabbit mAb Cell Signaling Technology Cat# 5869, RRID:AB_10707365 phospho-Ulk1 (Ser757) pAb Cell Signaling Technology Cat# 6888, RRID:AB_10829226 Anti-Laminin-gamma-2 (E 6) mAb Santa Cruz Biotechnology Cat# sc-28330, RRID:AB_2134476 Anti-Col1a1 (H-197) rabbit pAb Santa Cruz Biotechnology Cat# sc-28657, RRID:AB_2229646 Anti-F4/80 (D2S9R) XP Rabbit mAb Cell Signaling Technology Cat# 70076, RRID:AB_2799771 Anti-CCL2 mouse mAb (Clone T202) Creative Diagnostics Cat# CABT-35128MH, RRID:AB_2356195

Recombinant:

Article Title: RAB3B Dictates mTORC1/S6 Signaling in Chordoma and Predicts Response to mTORC1‐Targeted Therapy
Article Snippet: .. Product name Company Product code Anti-S6K1 antibody Abcam ab32529 Anti-S6K1 (phospho T389) antibody Abcam ab126818 S6 Ribosomal Protein Rabbit mAb Cell Signaling Technology #2217 S6 Ribosomal Protein (54D2) Mouse mAb Cell Signaling Technology #2317 Phospho-S6 Ribosomal Protein (Ser235/236) Rabbit mAb Cell Signaling Technology #4858 Phospho-S6 Ribosomal Protein (Ser240/244) Rabbit mAb Cell Signaling Technology #5364 p70 S6 Kinase Antibody Cell Signaling Technology #9202 Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb Cell Signaling Technology #9234 Anti-RSK1 p90 antibody Abcam ab32114 Anti-RSK1 (phospho S380) p90 antibody Abcam ab32203 Phoshpo-p90RSK (Thr359/Ser363) Antibody Cell Signaling Technology #9344 RSK2 Recombinant Rabbit Monoclonal Antibody HUABIO JE63-19 RSK3 Polyclonal antibody Proteintech 14446-1-A PKAα/β/γ cat Rabbit Polyclonal Antibody HUABIO ER64618 Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) Antibody Abcam Ab75991 DUSP12 Monoclonal Antibody Proteintech 67101-1-Ig DUSP12 Polyclonal antibody Proteintech 15667-1-AP PPM1B Polyclonal antibody Proteintech 13193-1-AP Akt Antibody Cell Signaling Technology #9272 Phospho-Akt (Ser473) Rabbit mAb Cell Signaling Technology #4060 Phospho-Akt (Thr308) (D25E6) XP Rabbit mAb Cell Signaling Technology #13038 p44/42 MAPK (Erk1/2) Antibody Cell Signaling Technology #9102 Phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (D13.14.4E) XP Rabbit mAb Cell Signaling Technology #4370 Anti-mTOR (phospho S2448) antibody Abcam ab109268 mTOR Rabbit mAb Cell Signaling Technology #2983 Phospho-4E-BP1 (Thr37/46) Rabbit mAb Cell Signaling Technology #2855 Anti-Nanog antibody Abcam ab109250 NANOG Rabbit pAb ABclonal Technology A14150 SOX2 (D1C7J) XP Rabbit mAb Cell Signaling Technology #14962 Anti-OCT4 antibody Abcam ab19857 Anti-Brachyury/Bry antibody Abcam ab209665 RAB3B/C Polyclonal Antibody Proteintech 15774-1-AP RAB3B monoclonal antibody (M02), clone 1A7 Abnova H00005865- M02 Anti-beta Tubulin antibody Hangzhou Bio Technology db3285 Anti-Puromycin Antibody, clone 12D10 Sigma-Aldrich MABE343 Table S2. ..

Western Blot:

Article Title: CCN2-induced lymphangiogenesis is mediated by the integrin αvβ5–ERK pathway and regulated by DUSP6
Article Snippet: .. Target Antibody Supplier/ catalogue number phospho-ERK Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody Cell Signaling Technology / 9101 ERK (for WB) p44/42 MAPK (Erk1/2) Antibody Cell Signaling Technology / 9102 ERK (for IP and IHC) p44/42 MAPK (Erk1/2) (137F5) rabbit mAb Cell Signaling Technology / 4695 ERK (for ICC) p44/42 MAPK (Erk1/2) (L34F12) mouse mAb Cell Signaling Technology / 4696 phospho-p38 Phospho-p38 MAPK (Thr180/Tyr182) (3D7) rabbit mAb Cell Signaling Technology / 9215 p38 p38 MAPK Antibody Cell Signaling Technology / 9212 phosho-JNK Phospho-SAPK/JNK (Thr183/Tyr185) Antibody Cell Signaling Technology / 9251 JNK SAPK/JNK Antibody Cell Signaling Technology / 9252 phospho-AKT Phospho-Akt (Ser473) (D9E) XP rabbit mAb Cell Signaling Technology / 4060 AKT Akt (pan) (C67E7) rabbit mAb Cell Signaling Technology / 4691 phospho-NFkB Phospho-NF-kB p65 (Ser536) (93H1) rabbit mAb Cell Signaling Technology / 3033 NFkB NF-kB p65 (D14E12) XP rabbit mAb Cell Signaling Technology / 8242 HSP90 HSP90 Antibody Cell Signaling Technology / 4874 GAPDH GAPDH (14C10) rabbit mAb Cell Signaling Technology / 2118 phospho-Smad1/5/9 Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467) (D5B10) rabbit mAb Cell Signaling Technology / 13820 Smad1 Smad1 (D59D7) XP rabbit mAb Cell Signaling Technology / 6944 Smad5 Smad5 (D4G2) rabbit mAb Cell Signaling Technology /12534 phospho-Smad2 Phospho-Smad2 (Ser465/467) (138D4) rabbit mAb Cell Signaling Technology / 3108 Smad2 Smad2 (D43B4) XP rabbit mAb Cell Signaling Technology / 5339 phospho-Smad3 Phospho-Smad3 (Ser423/425) (C25A9) rabbit mAb Cell Signaling Technology / 9520 Smad3 Smad3 (C67H9) rabbit mAb Cell Signaling Technology /9523 Integrin av Anti-Integrin alpha V antibody [EPR16800] abcam / ab179475 Integrin a9 Anti-Integrin alpha 9 antibody [EPR9722] abcam / ab140599 Integrin b1 Anti-Integrin b1 Antibody, clone MB1.2 R&S systems / MAB1997 Integrin β3 Anti-Integrin beta 3 antibody [ERP17507] abcam / ab179473 Integrin β5 Mouse/Rat Integrin beta 5 Antibody R&S systems /AF8035 Integrin αVβ3 Anti-Integrin alpha V beta 3 antibody [LM609] abcam / ab190147 Integrin αVβ5 Anti-Integrin alpha V+beta 5 antibody [P1F6] abcam / ab1777004 DUSP6 Anti-DUSP6 antibody [EPR129Y] abcam / ab76310 LYVE-1 Rabbit Anti-Mouse Lyve-1 Relia Tech / 103-PA50AG Podoplanin Purified anti mouse Podoplanin Antibody Biolegend / 127401 CD31 Anti-Pecam-1 Antibody, clone 390 Merck / CBL1337 b3-Tubulin b3-Tubulin (D65A4) XP rabbit mAb Cell Signaling Technology / 5666 Supplementary Figure S1. ..

Article Title: CoQ Regulates Brown Adipose Tissue Respiration and Uncoupling Protein 1 Expression.
Article Snippet: .. Antibody Source Identifier beta Tubulin Abcam Cat# ab131205, RRID:AB_11156121 beta Actin Sigma-Aldrich Cat# A1978, RRID:AB_476692 UCP1a Abcam Cat# ab209483 RRID:AB_2722676 UCP1 (E9Z2V) XP Rabbit mAb Cell Signaling Technology Cat# 72298 Total OXPHOS Rodent WB Antibody Cocktail Abcam Cat# ab110413 IRDye 680LT Goat anti-Mouse IgG antibody LI-COR Biosciences Cat# 925-68020, RRID:AB_2687826 IRDye 800CW Goat anti-Rabbit IgG antibody LI-COR Biosciences Cat# 925-32211, RRID:AB_2651127 ..

Immunohistochemistry:

Immunocytochemistry:

Purification:

Chromatin Immunoprecipitation:

Article Title: Molecular mechanism of Activin receptor inhibition by DLK1
Article Snippet: .. Blinding is not relevant to the study as the output parameters are not subjective and therefore not subject to this form of bias. re p o rtfo lio | rep o rtin g su m m ary A pril2023 Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication Mycoplasma contamination Commonly misidentified lines (See ICLAC register) anti-hPref1 Alexa Fluor 488 Conjugated antibody, Mouse monoclonal, Clone # 211309, R&D Systems Cat# FAB1144G, RRID:AB_3645938 anti-Myc-Tag (9B11) Mouse mAb Alexa Fluor® 488 Conjugated Cell Signaling Technology Cat# 2279, RRID:AB_2151849 anti Myosin Heavy Chain (MF20) mouse R&D Systems Cat# MAB4470, RRID:AB_1293549 anti-Notch1 Antibody (A-8) mouse monoclonal Santa Cruz Biotechnology Cat# sc-376403, RRID:AB_11149738 anti-Smad2/3 (D7G7) XP Rabbit mAb Cell Signaling Technology Cat# 8685, RRID:AB_10889933 Goat Anti-Human IgG Fc-AF647, Southern Biotech, Cat# 2048-31, RRID:AB_2795692 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488, Thermo Fisher Scientific, Cat# A32723, RRID:AB_2633275 Rat anti-human IgG Fc ( clone M1310G05) Alexa Fluor(R) 488, BioLegend, Cat# 410705, RRID:AB_2565783 anti-hPref1 Alexa Fluor 488 Conjugated antibody is validated by vendor and same clone as hPref1-647. ..

Flow Cytometry:

Magnetic Resonance Imaging:

Biomarker Discovery:

Marker:

Article Title: Adipocyte-derived extracellular vesicles are key regulators of central leptin sensitivity and energy homeostasis.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GLP1R Polyclonal Antibody Thermo Fisher Scientific Cat# PA5-33590, RRID: AB_2550968 Mouse Leptin R Antibody R&D Systems Cat# AF497, RRID: AB_2281270 GFAP antibody [GF5] - Astrocyte Marker Abcam Cat# ab10062, RRID: AB_296804 Anti-Iba-1 Wako Cat# 019-19741 RRID: AB_839504 Anti-NeuN antibody [1B7] - Neuronal Marker Abcam Cat# ab104224, RRID: AB_10711040 PIAS3 (D5F9) XP Rabbit mAb Cell Signaling Technology Cat9042, RRID: AB_11178593 c-Fos (9F6) Rabbit mAb Cell Signaling Technology Cat# 2250, RRID: AB_2247211 TSG101 antibody Abcam Cat# ab125011, RRID: AB_10974262 CD9 antibody Abcam Cat# ab92726, RRID: AB_10561589 Dicer antibody Abcam Cat# ab14601, RRID: AB_443067 Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb Cell Signaling Technology Cat# 52075, RRID: AB_2799407 PTEN (138G6) Rabbit mAb Cell Signaling Technology Cat# 9559, RRID: AB_390810 Anti-SOCS3 antibody Abcam Cat# ab16030, RRID: AB_443287 Anti-TCPTP antibody [EPR28199-34] Abcam Cat# ab314496, RRID: AB_314496 CD97 antibody Abcam Cat# ab108368, RRID: AB_10865208 SIRPalpha/SHPS1 (D6I3M) Rabbit mAb Cell Signaling Technology Cat# 13379, RRID: AB_2798196 Mouse Anti-ITGA7 Monoclonal Millipore Cat# ST1637, RRID: AB_10696472 Anti-CD146 antibody Abcam Cat# ab75769, RRID: AB_2143375 Anti-rabbit IgG-HRP antibody Santa Cruz Biotechnology Cat# sc-2077, RRID: AB_631745 Anti-mouse IgG-HRP antibody Santa Cruz Biotechnology Cat# sc-2314, RRID: AB_641170 Anti-Rabbit IgG, Alexa Fluor 488 conjugated Thermo Fisher Scientific Cat# A-11034, RRID: AB_2576217 Anti-Mouse IgG, Alexa Fluor 488 conjugated Thermo Fisher Scientific Cat# R37120, RRID: AB_2556548 Anti-Chicken IgY, Alexa Fluor 594 conjugated Thermo Fisher Scientific Cat# A-11039, RRID: AB_2534096 Anti-Rabbit IgG, Alexa Fluor 647 conjugated Thermo Fisher Scientific Cat# A-31573, RRID: AB_2536183 Anti-Mouse IgG, Alexa Fluor 594 conjugated Thermo Fisher Scientific Cat# R37121, RRID: AB_2556549 Anti-Rabbit IgG, Alexa Fluor 594 conjugated Thermo Fisher Scientific Cat# R37117, RRID: AB_2556545 Bacteria and virus strains rAVV-CMV-palm-mCherry-WPRE-hGH pA BrianVTA N/A rAVV-CMV-mCherry-WPRE-hGH pA BrianVTA N/A rAAV-U6-shRNA(Dicer) -CMV-mCherrySV40 pA BrianVTA N/A rAAV-U6- shRNA(Scramble) -CMVmCherry-SV40 pA BrianVTA N/A rAAV-U6-shRNA(LepR) -CMV-mCherrySV40 pA BrianVTA N/A rAAV-U6- shRNA(Scramble) -CMVmCherry-SV40 pA BrianVTA N/A rAAV-U6-shRNA(MC4R) -CMV-mCherrySV40 pA BrianVTA N/A (Continued on next page) Cell Metabolism 38, 1–18.e1–e8, January 6, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER rAAV-U6- shRNA(Scramble) -CMVmCherry-SV40 pA BrianVTA N/A rAAV-hSyn-mCherry-miRNAs-WPREs BrianVTA N/A rAAV-hSyn-mCherry-Scramble-WPREs BrianVTA N/A rAAV-mCherry-miRNAs-WPREs BrianVTA N/A rAAV-mCherry-Scramble-WPREs BrianVTA N/A rAAV-miR-302f 3 ′ UTR-mCherry-WPREs BrianVTA N/A rAAV-mCherry- hsa_miR-302f -WPREs BrianVTA N/A rAAV-mCherry-Scramble-WPREs BrianVTA N/A rAAV-Cre-WPREs BrianVTA N/A rAAV-Control-WPREs BrianVTA N/A Chemicals, peptides, and recombinant proteins Leptin Noveprotein Cat# CR70 Insulin human Eli Lilly and Company Cat# NDC 0002-8215-17 Trizol LS Reagent Life Technologies Cat# 10296 TRIzol RNA isolation reagent ThermoFisher Scientific Cat# 15596026 PKH26 Sigma Cat# PKH26GL-1KT Triton X-100 Roche Cat# 11332481001 DAPI Beyotime Cat# C1002 60% high fat diet Research Diets Cat# D12492 RIPA buffer (10x) Cell Signaling Technology Cat# 9806 DMEM/F-12 Gibco Cat# 11320082 Lipofectamine RNAiMAX reagent ThermoFisher Scientific Cat# 13778-075 Sucrose Sigma-Aldrich Cat# S0389 Fetal bovine serum Gibco Cat# 16140-071 Critical commercial assays SYBR premix Ex TaqTM II kit Takara Cat# RR420A ECL Western Blotting Substrate Thermofisher Cat# 32106 RNAscope Multiplex Fluorescent V2 Assay ACDbio Cat# 323270 RNAscope Probe- Mm-Fos-O5 ACDbio Cat# 584741 RNAscope Probe- Mmu-MC4R ACDbio Cat# 535481 RNAscope Probe- Mm-Agrp ACDbio Cat# 400711 RNAscope Probe- Mm-Pomc ACDbio Cat# 314081 Mouse Ghrelin ELISA Kit Merck Cat# EZRGRT Multispecies GLP-1 ELISA Kit Thermofisher Cat# BMS2194 Mouse Leptin ELISA Kit Thermofisher Cat# KMC2281 Mouse Insulin ELISA Kit Thermofisher Cat# EMINS Experimental models: Cell lines HEK293 cell line ATCC CRL-11268 Experimental models: Organisms/strains Mouse: C57BL/6J Gem Pharmatech Cat# N000013 Mouse: CD63 Flag EGFP-mCherry f/f Gem Pharmatech N/A Mouse: Agrp-Cre Jax stock Cat# 012899 Mouse: Rosa26-LSL-tdTomato Gem Pharmatech Cat# T002249 Mouse: POMC-creER Gem Pharmatech N/A Mouse: C57BL/6J-db/db Gem Pharmatech Cat# T017651 Deposited data RNA sequence raw data GEO database GSE295693 and GSE95694 (Continued on next page) e2 Cell Metabolism 38, 1–18.e1–e8, January 6, 2026

Bacteria:

Virus:

shRNA:

Modification:

Article Title: Gut substrate trap of D-lactate from microbiota improves blood glucose and fatty liver disease in obese mice.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals and Reagents Sodium L-lactate Sigma Aldrich L7022 Sodium D-lactate Sigma Aldrich 71716 [3-13 C] sodium D-lactate Cambridge Isotope Laboratories CLM-10768-PK [3-13 C] sodium L-lactate Cambridge Isotope Laboratories CLM-1579-0.1 [C 14 ] D-lactate American Radiolabeled Chemicals ARC-0593-50 [C 14 ] L-lactate American Radiolabeled Chemicals ARC 3081-50 Collagenase from Clostridium histolyticum type IV Sigma Aldrich C5138 EGTA BioShop EGT202.50 HEPES Sigma/BioShop H3375/HEP001 William’s E medium Wisent 301-018-CL Dulbecco’s Modification Eagle’s Medium (DMEM) without glucose/phenol red/LGlutamine/Sodium Pyruvate gibco A14430-01 Fetal Bovine Serum (FBS) Wisent 098-150 Phosphate Buffered Saline (PBS) Wisent 311-010-CL Antibiotic-antimycotic mixture Wisent 450115EL L-glutamine Invitrogen 25031049 D-glucose Sigma Aldrich G7021-1KG Sodium pyruvate Sigma Aldrich P2256-5G NovoRapid® (Insulin) Novo Nordisk Canada DIN02244353 o-Dianisidine dihydrochloride Sigma Aldrich D3252-5G o-Phenylenediamine 98% Beantown Chemical H2116750-25G Hydrogen peroxide EMD HX0635 Potassium Phosphate Dibasic Sigma Aldrich P3786-500G Sulfuric Acid EM Science 7664-93-9 Hydrochloric Acid Sigma Aldrich 258148–2.5L Sodium Hydroxide Sigma Aldrich 221465-500G Isopropanol VWR BDH Chemicals BDH1133-1LP Bovine collagen type I Sigma Aldrich C9879-1G Chloramine T Trihydrate Sigma Aldrich 402869-100G 4-(Dimethylamino)benzaldehyde (MDAB) Sigma Aldrich 156477-25G Hexadecyltrimethylammonium bromide (CTAB) Bio Basic Inc. CB0108 Acetic acid Glacial Caledon Laboratories Inc. 1000-1-29 Citric acid Caledon Laboratories Inc. 3020–1 Sodium acetate Sigma Aldrich S2889 Sodium citrate, dihydrate BioShop CIT001 Tween20 Sigma Aldrich P1379 Hematoxylin Sigma Aldrich GHS232 Ammonium hydroxide solution Sigma Aldrich 320145 Lithium carbonate, ACS, for MI Sigma Aldrich 62470 Lactobacillus MRS Broth w/o Dextrose Cepham Life Sciences 66416-0 Ampicillin trihydrate Sigma Aldrich A6140 (Continued on next page) Cell Metabolism 37, 1–14.e1–e7, September 2, 2025 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Neomycin trisulfate salt hydrate Sigma Aldrich N6836 Ultrapure LPS from E. coli (O111:B4) InvivoGen TLRL-3PELPS D-Glucose (1-13 C, 98-99%) Cambridge Isotope Laboratories Inc. CLM-420-0.25 Lactulose (U-13 C 12 , 99%) Cambridge Isotope Laboratories Inc. CLM-9043-PK [1-13 C fru ] lactulose Omicron LAC-009 Dulbecco’s Modification Eagle’s Medium (DMEM) with 4.5 g/L glucose and 2 mM l-glutamine Wisent 319-051 Penstrep Wisent 450–201 Normocin InvivoGen ANT-NR-05 HEK Blue detection media InvivoGen HB-DET2 HEK Blue selection media InvivoGen HB-SEL PURASORB PL10 Corbion N/A PURASORB PL38 Corbion N/A PURASORB PL65 Corbion N/A PURASORB PL24 Corbion N/A PURASORB PD24 Corbion N/A PURASORB PDL20 Corbion N/A Critical commercial assays PierceTM BCA Protein Assay Kit Thermo Scientific 23225 AmpliteTM Colorimetric D-Lactate Dehydrogenase (LDH) Assay Kit AAT Bioquest 13809 Amplite® Colorimetric L-Lactate Dehydrogenase (LDH) Assay Kit AAT Bioquest 13813 PicoProbe D-Lactate Assay Kit (Fluorometric) Abcam ab174096 Picoprobe L-Lactate Assay Kit (Fluorometric) Abcam ab169557 AmpliteTM Fluorimetric D-Lactate Assay Kit AAT Bioquest 13810 Amplite® Fluorimetric L-Lactate Assay Kit AAT Bioquest 13814 Glycogen Assay Kit II (Colorimetric) Abcam ab169558 Triglyceride Assay Kit Abcam ab65336 High sensitive mouse insulin immunoassay kit ImmunoDiagnostics 32270 VECTASTAIN Elite ABC-HRP Kit, Rabbit IgG BioLynx VECTPK6101 Antibodies F4/80 (D2S9R) XP Rabbit mAb Cell Signaling Technology Cat# 70076T; RRID: AB_2799771 α-smooth muscle actin (D4K9N) XP Rabbit mAb Cell Signaling Technology Cat# 19245T; RRID: AB_2857972 Experimental Models: Organisms/Strains Mouse: C57BL/6N Taconic Biosciences, Inc. RRID:IMSR_TAC:B6 Mouse: C57BL/6N Germ-free In-house colonies bred in the Farncombe Gnotobiotic Unit at the McMaster Animal Facilities N/A Mouse: Ob/Ob -/- The Jackson Laboratory RRID:IMSR_JAX:000632 Mouse: C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Lactobacillus reuteri I49 Gifted by Dr. Kathy McCoy (UCalgary) N/A Lactobacillus intestinalis ASF360 Gifted by Dr. Kathy McCoy (UCalgary) N/A Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 HEK-293T mTLR4 Cells InvivoGen HKB-mTLR4 (Continued on next page) e2 Cell Metabolism 37, 1–14.e1–e7, September 2, 2025 ..

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